Standards & reference frameworks

European standards & reference frameworksFrench standards & reference frameworks

European standards & reference frameworks


NF EN 1040

Determination of basic bactericidal activity — Phase 1

  • Pseudomonas aeruginosa – Staphylococcus aureus
  • Microbial reduction > 10⁵
  • Contact time: 1, 5, 15, 30, 45 or 60 minutes
  • 20°C


NF EN 1275

Determination of basic fungicidal activity

  • Candida albicans – Aspergillus niger
  • Microbial reduction > 10⁴
  • Contact time: 5, 15, 30 or 60 minutes
  • 20°C


NF EN 1276

Determination of bactericidal activity of disinfectants used in food, industrial, domestic and institutional sectors — Phase 2, Step 1

  • Pseudomonas aeruginosa – Escherichia coli – Staphylococcus hirae and other additional strains as optional
  • Microbial reduction > 10⁵
  • Contact time: 5 minutes and other additional times as optional
  • 20°C and other additional temperatures as optional
  • Dirty conditions: 3 g/L bovine albumin
  • Clean conditions: 0.3 g/L bovine albumin

French standards & reference frameworks


NF T 72-150 or T 72-151

Determination of bactericidal activity — Spectrum 4 or Spectrum 5

  • Pseudomonas aeruginosa – Escherichia coli – Staphylococcus aureus – Enterococcus hirae (Mycobacterium smegmatis)
  • Microbial reduction > 10⁵
  • Contact time: 5 minutes
  • 20°C


NF T 72-170 or T 72-171

Determination of bactericidal activity — Spectrum 4 or 5 in the presence of interfering substances

  • Pseudomonas aeruginosa – Escherichia coli – Staphylococcus aureus – Enterococcus hirae (Mycobacterium smegmatis)
  • Microbial reduction > 10⁵
  • Contact time: 5 minutes
  • 20°C
  • Hard water: 60° French — Proteins: 1% albumin + 1% yeast extract — Buffer solutions pH 5 and pH 9 — Dirty conditions: 30° French hard water + 0.3% albumin


NF T 72-180

Determination of virucidal activity against vertebrate viruses

  • Enterovirus Polio I, Sabin strain – Human adenovirus type 5 – Vaccinia orthopoxvirus
  • Viral reduction > 10⁴
  • Contact time: 15, 30 and 60 minutes
  • 20°C


NF T 72-181

Determination of activity against bacteriophages

  • Bacteriophage T2 – Bacteriophage MS2 – Bacteriophage ΦX174 – Bacteriophage No. 66
  • Viral reduction > 10⁴
  • Contact time: 15 minutes
  • 20°C


NF T 72-190

Determination of bactericidal, fungicidal and sporicidal activity — Carrier method

  • Souches référencées dans les normes, temps de contact et température non fixés :
    • NF T 72-150 ou 151 Réduction microbienne >105
    • NF T 72-200 or 201: microbial reduction > 10⁴
    • NF T 72-230 or 231: microbial reduction > 10³


NF T 72-300 or T 72-301

Determination of product efficacy against various micro-organisms under practical conditions of use

  • Bacterial reduction > 10⁵
  • Yeast and mould reduction > 10⁴
  • Bacterial spore reduction > 10³
  • Contact time: not fixed
  • Temperature: not fixed
  • Interfering substances: 30° French hard water or variable according to conditions of use